NeOse Advance
Table of Contents
Table of Contents
NeOse Advance OverviewSetting Up the systemSensor Maintenance and Replacement for generation 1.5NeOse Advance Overview
NeOse Advance is an odor detection device that enables detection and recording of odors in near real-time. It is operated by Aryballe Suite, an application software bundle that executes protocols, allows for adjustment of experimental parameters, analyzes recorded data and provides reports.
With its two inlets, the NeOse Advance product allows for automated switching between the baseline (to measure the olfactive background at a given place and time) and the sample to be analyzed. The air taken in is directed through an internal fluidic system to the Core Sensor, where VOCs interact with Aryballe' proprietary biosensors immobilized on Mach-Zehnder Interferometers. The resulting optical signal is processed using Aryballe's algorithms to generate a unique odor signature.
The NeOse Advance uses 2 male Luer Lock type fluidic inlets (Baseline and Sample), sequentially flowing the air through the odor, temperature and humidity sensors. Having a separate inlet for baseline maintains a dedicated fluidic path that is maintained as clean as possible.
The baseline inlet of the NeOse Advance is connected to a removable PTFE filter to prevent environmental pollution from entering the device.
The NeOse Advance is powered through a USB cable connected to a computer Super-Speed USB port.
Setting Up the system
Fluidic Installation
To be performed for each use, before warming up
1. Unscrew the translucent caps covering the NeOse Advance inlets.
2. Place a non-sterile PTFE syringe filter (provided) on the NeOse Advance baseline inlet to protect the device from environmental pollution. To do so, gently push the female part (i.e. the shortest end) of the filter into the male part of the device and screw them together using the lock on the NeOse Advance Luer.
Important
When drawing in ambient air, always use a filter to protect the sensor from external particles and pollutions. The PTFE filter should remain on the baseline inlet at all times while the device is operating to avoid premature aging of the sensor.
See HeptaValve Mini Manual for accessory connection when using Start Up or Start Up Plus Bundles
See Amplifier Manual for accessory connection when using Max or Start Up Plus Bundles
Device Start Up & Shutdown
See Aryballe Suite Manual for Software Instructions
System Checks
Before starting any experiment, we strongly recommend performing a full system validation to ensure that the system is fully functional and provide traceability for future reference.
Two system check protocols have been designed: a cleanliness check to ensure that the fluidic paths are clean of any contamination, and a quality check to ensure that the NeOse Advance has a good performance level.
See Aryballe Suite Manual for Instructions
Sensor Maintenance and Replacement for generation 1.5
Changing the Core Sensor & Updating device's memory
Prerequisites:
- a clean workstation
- the hexagonal tip screwdriver provided by Aryballe
- the new core sensor inside its protective box
- a computer with Aryballe Suite installed and an Internet connection
-
Aryballe strongly advises wearing gloves to avoid polluting the system.
1. Open Aryballe Suite
2. Go to Settings (gear icon)
3. Click on “Start Core Sensor Swap”
The 5-characters number is displayed on the package and on the core sensor and on the CS itself.
5. Click on "Continue"
Note: if the serial number is wrong or the Internet connection is missing, you will be informed with an error message.
6. Step 2: Removing the core sensor
- Unplug the device for safety
- Flip the device and unlock NeOse Advance black front cover by unscrewing the 2 front screws located under the device using the hexagonal tip screwdriver.
- Remove NeOse Advance black front cover by lifting it from its side closest to the inlets.
d. Unscrew the locking system with black and white markings and lift the hood.
7. Step 3: Confirm that the Core sensor into the device is the one with the serial number previously entered.
a. Place the new Core sensor into the dedicated place.
b.Close back the hood
c. Screw the locking system with a black and white markings. Make sure it is tightly closed by making the black arrow pointing to the right-hand side as shown:
d. Click on "Continue".
8.Step 4:
a. Plug the device to the computer
b.Wait 2 seconds
c. Click on "Discover"
d. Once the device is connected, click on “Continue” to start the calibration.
9.Step 5: the device calibration will automatically start. Do not touch anything until completion.
10. Once the calibration is over, close back the black front of the device and screw it. You can start using the device as is. However, Aryballe advises running a Quality Check to ensure the quality of your next experiments.
Note: if the calibration ends with this error:
- Make sure the Core Sensor is secured within its support.
- Try moving the core sensor a bit before closing again the hood, and “Start again”
- If all tries are failing, contact the user support team with the email address.
Note: if you meet this error, this means the device was disconnected. Try unplugging then plugging the device and “Retry Calibration” with the same Core Sensor:
Sensor Maintenance and Replacement for generation 1.0
Changing the Core Sensor
Removing the old Core Sensor
The Core Sensor module appears as follows:
When changing the Core Sensor, make sure that the NeOse Advance is switched off to prevent the pumping of dust or particles into the device. The procedure to remove the module is the following:
1. Unlock the NeOse Advance black front cover by unscrewing the 2 front screws located under the device using the hexagonal tip screwdriver provided by Aryballe.
3. Release the tubing connecting the Core Sensor module to the humidity and temperature sensor by pressing the black rim of the push in fitting stud while pulling the tubing out.
4. Release the tubing connecting the Core Sensor module to the internal sampler by completely unscrewing the beige connector.
5. Disconnect the flat ribbon cable from the electronic broad by pressing on both sides of the black connector simultaneously and gently moving it laterally and upwards to remove. Do not disconnect the cable on the Core Sensor directly.
6. Completely unscrew the 4 screws to release the Core Sensor from its black support.
7. Remove the Core Sensor from NeOse Advance.
Installing the new Core Sensor
1. Place the new Core Sensor in the empty Core Sensor location and replace the 4 screws to fix the Core Sensor onto its support.
3. Reconnect in the tubing connecting the Core Sensor module to the internal sampler by screwing the beige connector.
4. Reconnect the humidity and temperature sensor tubing into the black push in fitting stud by pushing it so that the Core Sensor module is strongly connected to the NeOse Advance fluidic system.
5. Put the NeOse Advance front cover back in place by first inserting the black tabs into the device white cover.
6. To affix the case cover and complete the replacement process, screw the 2 screws located under the device using the provided screwdriver. Tighten the first screw halfway, then screw in the second screw before completely tightening both screws.
Removing the whole system
The whole system module can be seen in the following photo. It encompasses the Core Sensor module as well as the NeOse Advance Internal Sampler block. Replacement of the whole system can be warranted in case of contamination of the fluidic system or valve failure.
When changing the whole system, make sure that NeOse Advance is switched off to prevent the pumping of dust or particles into the device. The procedure to remove the module is the following:
1. Unlock NeOse Advance black front cover by unscrewing the 2 front screws located under the device using the hexagonal tip screwdriver provided by Aryballe.
2. Remove NeOse Advance black front cover by lifting it from its side closest to the inlets.
3. Release the tubing connecting the Core Sensor module to the humidity and temperature sensor by pressing the black rim of the push in fitting stud while pulling the tubing out.
4. Release the tubing connecting the Core Sensor module to Baseline inlet by pressing the black rim of the push in fitting stud while pulling the tubing out.
5. Release the tubing connecting the Core Sensor module to Sample inlet by pressing the black rim of the push in fitting stud while pulling the tubing out.
6. Disconnect the flat ribbon cable from the Aryballe electronic broad by pressing on both sides of the black connector simultaneously and gently moving it laterally to remove. Do not disconnect the cable on the Core Sensor directly.
7. Disconnect the two red and black double cables from the internal sampler by pulling gently the black connectors from the two metallic valves.
8. Completely unscrew the large black screw to release the whole system module from its support.
9. Remove the module from the NeOse Advance.
Installing the new whole system
1. Place the new whole system module in the empty module location.
2. Replace the large black screw to fix the module onto its support.
3. Reconnect the two red and black double cables to both valves of the internal sampler. There is no polarity for the valves, the orientation of the red or black cable is not important.
However, it is important to connect the red and black cable with the black tape to the valve with the black sticker to correctly define the Baseline and Sample inlets.
4. Reconnect the flat ribbon cable to the Aryballe electronic board, holding it with its black connector. Make sure to insert it straight into the metal connector. Do not hold it by the cable surface itself.
5. Reconnect the tubing for the Sample inlet to the Core Sensor module by pushing it into the nearest black push. Make sure that it is pushed as far as possible so that the Core Sensor module is tightly connected.
6. Reconnect the tubing for the Baseline inlet to the Core Sensor module by pushing it into the closest black push. Make sure that it is pushed as far as possible, so that the Core Sensor module is tightly connected.
7. Reconnect the humidity and temperature sensor tubing into the black push in fitting stud by pushing it so that the Core Sensor module is strongly connected to the NeOse Advance fluidic system.
8. Put the NeOse Advance front cover back in place by first inserting the black tabs into the device white cover.
9. To affix the case cover and complete the replacement process, screw the 2 screws located under the device using the provided screwdriver. Tighten the first screw halfway, then screw in the second screw before completely tightening both screws.
Replacing the internal sampler’s fluidics
1. Release the tubing connecting the Core Sensor module to the Baseline and Sample inlets by pressing the black rim of the push in fitting stud while pulling the tubing out.
2. Unscrew the two bolts of the Baseline and Sample inlets to release the two Male Luer Locks using a 10 mm wrench (not provided).
3. Remove the old PTFE tubing and install the new system.